What is horse serum proteins?

What is SAA in horses?

Serum amyloid A (SAA) is the major acute phase protein in horses. It is produced during the acute phase response (APR), a nonspecific systemic reaction to any type of tissue injury. In the blood of healthy horses, SAA concentration is very low, but it increases dramatically with inflammation.

Can agarose gel support serum protein electrophoresis in horses?

Conclusion: Using agarose gel as the supporting matrix for serum protein electrophoresis in horses resulted in excellent resolution and accurate results that facilitated standardization into 6 protein fractions. Animals Blood Proteins / analysis* Electrophoresis, Agar Gel / veterinary*

Is serum protein electrophoresis a useful screening test in Equine Laboratory Medicine?

1 Clinical Laboratory, Vetsuisse Faculty, University of Zurich, Zurich, Switzerland. Background: Serum protein electrophoresis is a useful screening test in equine laboratory medicine.

What is the lifeassays ® equine SAA test?

The LifeAssays ® Equine SAA Test is fast and easy. Use this quantitative and accurate test before the race or training session, or in regular veterinary practice to pick up on a systemic inflammation. Rule in/rule out inflammatory disease. Determine the extensiveness of an inflammation.

What does SAA mean in horse blood?

In the blood of healthy horses, SAA concentration is very low, but it increases dramatically with inflammation. Due to the short half-life of SAA, changes in its concentration in blood closely reflect the onset of inflammation and, therefore, measurement of SAA useful in the diagnosis and monitoring of disease and response to treatment.

Why do we need a rapid test for equine SAA?

Horses are an important part of many people’s lives and a big industry in many regions. We need them as much as they need us. That is why LifeAssays ® AB has developed a unique patented rapid test for Equine SAA.

What is SAA and why is it important?

– The Horse What is SAA, and Why is it Important to Equine Medicine? Recently, a biomarker called SAA has become a buzzword, garnering attention from the equine veterinary community for its ability to indicate inflammation. So just what is SAA and why are so many veterinarians and researchers starting to analyze it?

Does exercise affect serum protein levels in equine serum?

The results of our current study showed that exercise has a statistically non-significant effect on the total proteins and their fractions in equine serum. The fractions and the A/G ratio were within the range of values obtained in horses in other studies.

What is the difference between globulin and albumin in horse serum?

Albumin is the main (48-76% of TP) and the most osmotically active protein fraction of a horse serum (Winnicka, 2011) whereas a globulin fraction is a heterogeneous group of blood proteins including carrier proteins, enzymes, immunoglobulins and other inflammatory molecules (Abeni et al., 2013).

What is protein electrophoresis used for in horses?

Background: Serum protein electrophoresis is a useful screening test in equine laboratory medicine. The method can provide valuable information about changes in the concentrations of albumin and alpha-, beta-, and gamma-globulins and thereby help characterize dysproteinemias in equine patients.

Which serum proteins are high in serum protein in horses?

In the present work, albumin represented the highest percentage of serum proteins, followed by γ-globulin, β2a-globulin, β1-globulin, β2b-globuin and α1 and α2 fractions. In a study using 126 horses, the highest levels were also found in albumin and γ-globulin, followed however by α2, β1 and β2 globulin, and α1 (Riond et al. 2009 ).

What is serum protein electrophoresis?

Serum protein electrophoresis is a common technique of laboratory diagnosis in human, and has been studied intensively in small animal and equine medicine (Riond et al., 2009). However, in bovine medicine, the electrophoretic separation of serum proteins is a rarely used laboratory method.

What is a lifeassays test for horses?

Intended Use. The LifeAssays® Equine serum amyloid A (SAA) Test Kit is an in vitro veterinary diagnostic test for the quantitative determination of amyloid A in equine serum. It is intended to be used as an aid in the diagnosis, monitoring and therapy of various inflammatory conditions in horses.

What is the lifeassays equine serum SAA test kit?

The LifeAssays® Equine serum amyloid A (SAA) Test Kit is an in vitro veterinary diagnostic test for the quantitative determination of amyloid A in equine serum. It is intended to be used as an aid in the diagnosis, monitoring and therapy of various inflammatory conditions in horses.

What does SAA mean in blood test?

SAA is a so-called positive acute phase protein, i.e. it increases when the inflammatory system is stimulated. The concentration of SAA in serum or plasma is thus increased by: Anytime. Acute inflammation? The LifeAssays ® Equine SAA Test is fast and easy.

What is SAA and why is everyone talking about it?

Recently, a biomarker called SAA has become a buzzword, garnering attention from the equine veterinary community for its ability to indicate inflammation. So just what is SAA and why are so many veterinarians and researchers starting to analyze it? Every few years a new buzzword begins circulating in the horse health industry.

What is SAA and how does it affect your liver?

It’s a biomarker protein produced in the liver in the face of inflammation caused by infection. Dr. David Levine: SAA has several roles in inflammatory processes, but most importantly for us it serves as a marker for inflammation that increases and decreases quickly so it can give us real-time information using a blood test.

What is the difference between SAA and fibrinogen?

These include serum amyloid A (SAA) and fibrinogen. SAA rises quickly in response to inflammation or infection and will quickly return to normal levels following resolution of the inflammation or infection. Fibrinogen will rise in response to tissue damage.

How do you test globulin levels in horses?

Levels of these fractions can be measured using a process called serum protein electrophoresis. Increased globulin levels may be seen when a horse is fighting an infection. When newborn foals have a blood test to ensure adequate colostrum transfer from the mare levels of gamma globulin are measured.

What is equine glucose?

Glucose – Glucose is the source of the body’s energy. It is measured in suspected cases of equine metabolic syndrome and sometimes in cases of equine Cushing’s disease. Blood glucose may also be measured as part of a glucose tolerance test, assessing small intestinal function.

What is a high protein level in a horse’s serum?

Forty horses (48%) had serum protein concentrations above the maximum reference value (7.7 g/dL). The increase in serum protein concentration was associated with hyperglobulinemia (P = .00005, R2 = .80).

What does globulin do for a horse?

Globulin – Globulin proteins carry out a number of tasks, including assisting with immune function. Total globulin is made up of three fractions, alpha, beta and gamma globulin. Levels of these fractions can be measured using a process called serum protein electrophoresis. Increased globulin levels may be seen when a horse is fighting an infection.

Why is my horse’s albumin concentration so high?

Albumin concentration increases in excited horses and those suffering from haemoconcentration/dehydration. Albumin will also be higher in heparinized plasma samples than serum. Albumin will decrease with overhydration, decreased production (inanition, advanced hepatic disease, malabsorption,…

What are the components of an electrophoretogram?

The electrophoretogram splits the protein fraction of serum or plasma into its constituent components this includes albumin and globulins. There are three globulin fractions: alpha, beta and gamma.

What is total protein electrophoresis used for?

Total Protein Electrophoresis This procedure separates the proteins in serum and body fluids (e.g. peritoneal fluid, urine) into the component albumin and globulins. Electrophoresis is indicated for determination of the underlying nature of a hyperglobulinemia or if multiple myeloma is suspected in a patient.