Can I use FBS instead of horse serum?

Can you feed a horse hay instead of fiber?

The fiber content should be at least 15 percent (more is better) if no hay is fed. Complete feed has more calories per pound than hay, so feed according to label recommendations and not as a pound-for-pound substitution. However, it will not satisfy a horse’s need to chew.

How much FGF does horse serum increase cell growth?

When plated on fibronectin-coated dishes and exposed to varying concentrations of horse serum in the presence of 100 ng/ml fibroblast growth factor (FGF), increasing cell growth was observed up to a serum concentration of 50%. When 50% fetal bovine serum was used instead of horse serum proliferation was less.

Should fetal bovine serum be used instead of horse serum?

When 50% fetal bovine serum was used instead of horse serum proliferation was less. In contrast, bovine adrenocortical cells showed a maximal proliferative response to either fetal bovine serum or horse serum at 10%.

Can I substitute heat-inactivated horse serum for trypanosomatidaes?

In our laboratory Trypanosomatidaes (Trypanosoma evansi and Trypanosoma equiperdum) are grown with the use of heat-inactivated horse serum and this is also useful in stramenopile cultures Blastocystis spp. In principle you could try the substitution that you indicate. You need to perform a pilot test with a small volume of culture medium.

Is soaked fibre better for horses than hay?

As a soaked fibre feed takes considerably less effort and time to eat than the equivalent amount of hay, it is important to try to maximise the amount of time a horse spends eating to avoid long periods when no fibre is passing through the gut. A horse’s feeding time can be extended by:

Can horses eat grain instead of hay?

Although they can eat grain, they still need a forage substitute that provides the +20% fiber content that good pasture or hay provides. There are a few things that you can feed horses as a substitute for hay.

How much fiber should be in a horse feed?

The fiber content should be at least 15 percent (more is better) if no hay is fed. Complete feed has more calories per pound than hay, so feed according to label recommendations and not as a pound-for-pound substitution.

Why do horses gain weight so easily?

These horses tend to gain weight easily, put down unusual amounts of fat in unusual places, develop fun conditions like laminitis, and, in short, are “easy keepers” for a reason. However, that reason is not a lack of thyroid hormone. Remember your horse’s metabolism?

What grows when you use horse serum?

Trypanosoma evansi or Trypanosoma equiperdum grow when you use horse serum. Blastocystis spp. it adapts well to fetal bovine serum or horse serum.

How much horse serum should be added to cell line?

However, ATCC suggests to add horse serum upto 2.5%. Now the problem is that, cells have not been adhered to the flask surface even after 24 hr. Cell line doubling time is 40hr. Whether this will affect on the cell adherence.

How much Fe does a horse need to grow?

According to the 2007 Horse NRC, Fe requirements are 50 ppm for growing foals, lactating and pregnant mares, and 40 ppm for all other classes of horses. The 2005 NRC suggested a maximum tolerable Fe concentration of 500 ppm using data from other species.

Is it possible to use a horse serum as a culture medium?

Horse serum is mainly used in case of some cells-especially in differentiation media. As many suggested, it will be better to maintain the recommended culture conditions if you need to maintain the identity of the lines.

Is fetal bovine serum a good source of mesenchymal stromal cells?

Culture and expansion of equine mesenchymal stromal cells (MSCs) are routinely performed using fetal bovine serum (FBS) as a source of growth factors, nutrients, and extracellular matrix proteins. However, the desire to minimize introduction of xenogeneic bovine proteins or pathogens and to standard …

Do bovine adrenocortical cells respond differently to fetal serum and horse serum?

In contrast, bovine adrenocortical cells showed a maximal proliferative response to either fetal bovine serum or horse serum at 10%.

What is the best serum for Blastocystis and Trypanosoma?

You can work in the case of Blastocystis with fetal bovine serum or with horse serum. There is a group of Peruvian researchers who argue that horse serum is better. In the laboratories where I worked with Trypanosoma cruzi they used only fetal bovine serum. Trypanosoma evansi or Trypanosoma equiperdum grow when you use horse serum.

Is horse serum better than human serum for Trypanosoma?

There is a group of Peruvian researchers who argue that horse serum is better. In the laboratories where I worked with Trypanosoma cruzi they used only fetal bovine serum. Trypanosoma evansi or Trypanosoma equiperdum grow when you use horse serum.

How do you filter Chelex-treated horse serum?

Sterile filter the chelex-treated horse serum through the pre-rinsed 0.2 micron filter. Aseptically aliquot into 5 x 100 mL quantities. Hope this will be useful. Selvam. Can I replace the goat serum by horse serum or bovine serum? Can I replace the goat serum by horse serum or bovine serum? Growing MDA-MB-231 and MDA-MB-4175 in Horse Serum?

Should you soak hay before feeding your horse?

Feeding hay that’s been soaked for at least an hour is beneficial if your horse is on a restricted diet, struggles with laminitis or a metabolic disease, or has to have a reduced-sugar diet. If your horse requires dust-free forage but needs the extra nutrients and energy that would be lost through soaking, haylage is an ideal solution.

Is soaked fibre feed better for horses?

As a soaked fibre feed takes considerably less effort and time to eat than the equivalent amount of hay, it is important to try to maximise the amount of time a horse spends eating to avoid long periods when no fibre is passing through the gut.

Why feed high fibre low starch for horses?

Benefits of a High Fibre, Low Starch Diet for Horses. Horses are trickle feeding herbivores that have evolved to consume a diet rich in structural carbohydrates (fibre), but low in soluble polysaccharides (starch). Fibre sources such as hay, haylage and grass are vital for a healthy digestive system and should always form the majority of the diet.

What is the best source of fibre for horses?

Fibre sources such as hay, haylage and grass are vital for a healthy digestive system and should always form the majority of the diet. Fibre also provides the horse with excellent levels of slow release energy and a good source of calories and heat as it is fermented in the body.