Are zebra danios aggressive fish?

What factors affect the fertilization rate of zebrafish (Danio rerio)?

Meinelt T. Schultz C. Worth M. Kurzinger H. Steinberg C. Dietary fatty acid composition influences the fertilization rate of zebrafish (Danio rerio Hamilton-Buchanon) J Appl Ichthyol. 1999;15:19–23. [ Google Scholar] 16.

Do standardized diets support zebrafish (Danio rerio) development?

The need to develop standardized diets to support zebrafish (Danio rerio) research is supported by the knowledge that specific dietary ingredients, nutrients, or antinutritional factors in diets have been shown to affect development and growth of adult D. rerio and their offspring.

Can sperm injections induce fertilization and embryonic development in zebrafish?

Our data also suggest that injections will be more likely to fertilize an egg at 5–40 min after collection. Thus, although we were able to induce fertilization and embryonic development by sperm injection in zebrafish, the efficiency was low.

Do standardized diets support zebrafish development?

The need to develop standardized diets to support zebrafish ( Danio rerio) research is supported by the knowledge that specific dietary ingredients, nutrients, or antinutritional factors in diets have been shown to affect development and growth of adult D. rerio and their offspring.

Do defined and undefined diets improve breeding success in zebrafish?

While future studies should focus on diets that enhance breeding by comparing the cross success rate, this investigation and corresponding results presented clearly demonstrate to the zebrafish community the importance of feeding defined diets and the potential experimental problems that may arise from utilizing undefined diets.

What is the effect of dietary HUFA on reproductive performance in zebrafish?

Influence of dietary HUFA levels on reproductive performance, tissue fatty acid profile and desaturase and elongase mRNAs expression in female zebrafish Danio rerio. Aquaculture. 2008; 277 :275–281. [ Google Scholar] [ Ref list] 15.

Can zebrafish fertilize eggs from wild-type pigmented males?

Only sperm from wild-type pigmented males were injected into eggs of two zebrafish lines (wild-type, pigmented and gold, long-fin). No significant difference was observed in fertilization rate between the two different lines (P = 0.997, logistic regression) and the data were combined. All embryos that developed after 48 h were pigmented.

Why study cardiovascular development in zebrafish embryos?

Zebrafish embryos are particular well suited for studying gene function during cardiovascular development, because zebrafish embryos are not completely dependent on a functional cardiovascular system for their development.

Can zebrafish be used to study human neurodegenerative diseases?

In recent years, zebrafish (Danio rerio) and medaka (Oryzias latipes) have become popular in pathogenic and therapeutic studies about human neurodegenerative diseases due to their small size, the optical clarity of embryos, their fast development, and their suitability to large-scale therapeutic screening.

Why are zebrafish so popular?

Well zebrafish are like that new guy at school who is kind of weird but in a cool way so everybody wants to hang out with him. Zebrafish are relatively new to the laboratory animal scene and their popularity has increased dramatically in the last 15 years, which has resulted in certain husbandry aspects being left in the dust.

Why do zebrafish need vitamin C?

Zebrafish, like other species of fish, require a dietary source of Vitamin C (ascorbic acid or dehydroascorbic acid) due to the lack of an enzyme necessary to synthesize it from glucose. This requirement is the foundation for the use of zebrafish as a model for vitamin C deficiency, a benefit that the rat or the mouse cannot confer.

How does dietary fiber affect gut health in zebrafish?

The only study looking at the impact of dietary fiber content in zebrafish showed that increased cellulose content from 1.5%–60% resulted in increased gut length, gut mass, surface area, and activity level of digestive enzymes, but decreased body mass ( Leigh, Nguyen-Phuc, & German, 2018 ).

What is the breeding protocol for wild zebrafish?

Wild-type AB strain adult zebrafish (Danio rerio, 5 months old) were purchased from a commercial dealer (Wuhan, China). According to the standard zebrafish breeding protocol (Tsang et al., 2017), all fish were maintained at 28 • C with a 14:10 h day/night cycle and fed freshly hatched brine shrimps two times per day.

Is α-tocopherol necessary for fetal resorption in zebrafish fed vitamin E-deficient diets?

Zebrafish (Danio rerio) fed vitamin E-deficient diets produce embryos with increased morphologic abnormalities and mortality Vitamin E (α-tocopherol) is required to prevent fetal resorption in rodents. To study α-tocopherol’s role in fetal development, a nonplacental model is required.

Do vitamin E-deficient diets affect embryo development in zebrafish?

Therefore, the zebrafish, an established developmental model organism, was studied by feeding the fish a defined diet with or without adde … Zebrafish (Danio rerio) fed vitamin E-deficient diets produce embryos with increased morphologic abnormalities and mortality

Does diet affect spawning in zebrafish (Danio rerio)?

Diet affects spawning in zebrafish. Influence of Commercial and Laboratory Diets on Growth, Body Composition, and Reproduction in the Zebrafish Danio rerio. Preliminary evaluation on the effects of feeds on the growth and early reproductive performance of zebrafish (Danio rerio).

Can sperm from zebrafish be injected directly into fish eggs?

To evaluate the potential for fertilization by sperm injection into fish eggs, sperm from zebrafish, Danio rerio, were microinjected directly into egg cytoplasm of two different zebrafish lines.

Do gametes from different zebrafish lines differ in fertilization?

The different gamete treatments were evaluated using eggs from two different zebrafish lines (gold, long-fin and wild-type, pigmented) and sperm from wild-type pigmented males. There was no significant difference in fertilization by ICSI between the two zebrafish lines.

Do nutritional factors affect reproductive performance in fish?

Indeed, one of the major nutritional factors that has been found to significantly affect reproductive performance in fish is the dietary essential fatty acid content Watanabe et al., 1984a, Watanabe et al., 1984b.

What is HUFA in fish food?

Highly unsaturated fatty acids (HUFA) with 20 or more carbon atoms affect, directly or through their metabolites, fish maturation and steroidogenesis. In some species, HUFA in broodstock diets increases fecundity, fertilization and egg quality.

How does HUFA affect egg quality?

In some species, HUFA in broodstock diets increases fecundity, fertilization and egg quality. As in higher vertebrates, vitamin E deficiency affects reproductive performance, causing immature gonads and lower hatching rate and survival of offspring.

Can probiotics increase fecundity in zebrafish?

Gioacchini, G., Maradonna, F., Lombardo, F., Bizzaro, D., Olivotto, I., & Carnevali, C., (2010a). Increase of fecundity by probiotic administration in zebrafish ( Danio rerio ).

Is the zebrafish a good model for studying vascular development?

In recent years the zebrafish has emerged as an important vertebrate model system for studying vascular development.

Why study heart disease in zebrafish embryos?

Zebrafish embryos have been extensively used to study heart development and cardiac function, mainly due to the unique embryology and genetics of this model organism. Since most human heart disease occurs during adulthood, adult zebrafish models of heart disease are being created to dissect mechanisms of the disease and discover novel therapies.

Is it possible to quantify cardiac function in zebrafish?

However, due to its small heart size, the use of cardiac functional assays in the adult zebrafish has been limited. To address this bottleneck, the transparent fish line casper;Tg (cmlc2:nuDsRed) that has a red fluorescent heart can be used to document beating hearts in vivo and to quantify cardiac functions in adult zebrafish.